Investigation of the Effects of Autophagy Signaling on the Transcription of Yeast Retrotransposon Ty2-917

Objective: Ty2-917 is a low copy retrotransposon found in the Saccharomyces cerevisiae genome. It has structural similarities to metazoan retroviruses terms of genome organization and propagation mechanisms host cells. The objective this study analyze effects autophagy signaling on transcriptional regulation Ty2 yeast Materials Methods: Ty2-LacZ gene fusions YEp vectors have been used as reporter genes amino acid starvation, nitrogen source, signals transcription Ty2. These transformed into wild type also isogenic mutant strains that are defective for one regulatory factors involved nutrient sensing signaling. To activate signaling, transformants were treated with caffeine or 3-amino 1-2-3 triazole. Transcription levels untreated cells analyzed by β-galactosidase assays. Results: Results show decreases up eightfold response starvation. Caffeine treatment represses transcription, independent TOR1 pathway. In addition, our results suggest regulated source-dependent manner through GATA factors. Conclusions: Our activation signal significant level repression transcription. We class